机构地区: 西北大学
出 处: 《生物技术通讯》 2005年第3期268-270,共3页
摘 要: 通过重叠区扩增法人工设计和合成一种新型抗菌肽CecropinB基因,按正确的阅读框架定向克隆至巴斯德毕赤酵母的高效表达载体pPIC9K上。经PCR鉴定及序列分析,所转化的大肠杆菌DH5α菌落中含有插入CecropinB基因的重组质粒pPIC9K-CB,表明成功构建了新型抗菌肽CecropinB表达载体pPIC9K-CB。 A novel antibacterial peptide cecropin B gene was designed and artificially synthesized through using gene splicing by overlap extension (gene SOEing) method. After this cecropin B gene was subcloned into plasmid pPIC9K, a novel recombinant expression plasmid pPIC9K-CB, which can be efficiently expressed in Pichia pastoris, can be obtained. By using PCR and sequence analyses, it can be proved that all the tested clones contain this novel cecropin B gene, and the novel cecropin B expression plasmid pPIC9K-CB is successfully constructed.
关 键 词: 抗菌肽 真核表达载体 构建 巴斯德毕赤酵母 高效表达载体 鉴定 人工设计 定向克隆 序列分析 大肠杆菌 重组质粒 基因 重叠区 菌落