作　　者： ; ; ; ; ;

机构地区： 山西农业大学动物科技学院

出　　处： 《中国农学通报》 2005年第5期1-4,共4页

摘　　要： 研究了关中奶山羊骨髓间充质干细胞的分离、培养和生物学特性,并在体外诱导分化为心肌细胞表型。结果表明,山羊MSCs具有很好增殖能力;能够耐受反复冷冻和解冻;表达细胞表面标志CD44、CD105、CD166,弱表达CD34、CD106,而不表达CD14、CD45。该类细胞用5-氮胞苷诱导分化后,能够表达心肌α-actin,并呈PAS染色阳性。 The Guanzhong dairy goats were used in our experiment to study the characteristics of their bone marrow mesenchymal stem cells (BM-MSCs), such as isolation, culture, and identification. The results indicate that there are excellent potentials for the goat mesenchymal-like stem cells derived from goat bone marrow to proliferate and to resist freezing and thawing repeatedly. The goat mesenchymal-like stem cells can express cellular surface markers CD34, CD44, CD105, CD106, CD 166, and TGF-α, but not CD14 and CD45. And after treated by 5-azacytidine in vitro, they express cellular surface marker of cardiac α-actin and Schiff's stain positive.

关 键 词： 诱导分化 干细胞生物学 心肌细胞 性能鉴定 骨髓间充质干细胞 关中奶山羊 生物学特性 氮胞苷 细胞表型 增殖能力 细胞表面 表达

领　　域： [生物学] [生物学]