作　　者： ; ; ; ;

机构地区： 华南农业大学动物科学学院

出　　处： 《中国家禽》 2005年第8期11-14,共4页

摘　　要： 95只1日龄SPF鸡随机分成5组,1～4组分别于10日龄接种ΦT4、ΦT4-Z1-HN、ΦT4-Z1-F、ΦT4-Z1-HN+ΦT4-Z1-F油乳剂疫苗,第5组接种ND弱毒疫苗,25日龄加强免疫一次。结果表明,重组HN和F蛋白均能诱导ND抗体产生,但抗体水平显著低于用弱毒疫苗免疫的第5组。60日龄时用100LD50NDV强毒株攻毒,第1组鸡100%发病和死亡,ΦT4-Z1-HN和ΦT4-Z1-F免疫组的死亡率分别为50%和15.8%;用ΦT4-Z1-HN+ΦT4-Z1-F和ND弱毒疫苗免疫的第4、5组鸡无一死亡。 95 1-day-old SPF chickens were randomly divided into 5 groups,The birds in groups 1,2,3 and 4 were respectively immunized with ΦT4,ΦT4-Z1-HN,ΦT4-Z1-F and ΦT4-Z1-HN+ΦT4-Z1-F oil-emulsion vaccine,and the chickens in group 5 were given attenuated ND viaccine,all birds were given a booster at the age of 25-days.The results showed that both of the recombinant phages ΦT4-Z1-HN and ΦT4-Z1-F were able to induce specific ND antibody, but the antiboby titers were significantly lower than that of the group 5.At 60 days of age,all birds were challenged with 100LD virulent NDV.When all birds in the group 1 became ill and exhibited typical ND symptoms, 50% and 15.8% mortality were observed in the groups vaccined with ΦT4-Z1-HN or ΦT4-Z1-F phage,The birds in group 4 and 5 immunized with ΦT4-Z1-HN+ΦT4-Z1-F and attenuated ND viaccine were protected against clinical disease and none of them died.

关 键 词： 重组新城疫病毒 蛋白 蛋白 免疫保护作用 基因工程疫苗 噬菌体展示系统

领　　域： [农业科学] [农业科学] [农业科学]