机构地区: 广西大学生命科学与技术学院广西亚热带生物资源保护利用重点实验室
出 处: 《广西农业生物科学》 2005年第1期18-22,42,共6页
摘 要: 从生物信息学角度,利用生物学数据库( Gen Bank、Swiss-Prot、PDB)和分子生物学分析软件VectorNTI8.0 ,对大肠杆菌K-1 2中的多个丙酮酸甲酸裂解酶( pyruvate formate-lyase,PFL)进行序列的比较分析和结构预测,进而分析其活性位点、相关保守位点和特殊结构域及序列的二级结构。 Sequence analysis and structure prediction of pyruvate formate-lyase ( PFL) were performed,based on the knowledge of bioinformatics,with the biological databases( Gen Bank,Swiss- Prot,PDB) and software( Vector NTI8.0 ) supported by Internet.The further analysis of active sites,conserved sites,motifs,and secondary structure of PFLs were obtained,which supply the informations forthe rational design to PFL.