作　　者： ; ; ; ; ; (屈仁建）;

机构地区： 华南农业大学兽医学院

出　　处： 《华南农业大学学报》 2005年第2期115-117,共3页

摘　　要： 用保守引物扩增鲁道夫对盲囊线虫Contracaecumrudolphii姊妹种和C .septentrionalerDNA第一内转录间隔区(ITS 1)片段并纯化,根据鲁道夫对盲囊线虫A、鲁道夫对盲囊线虫B和C .septentrionalerDNAITS 1序列,选用限制性内切酶MspΙ和NsiI酶切,酶切产物用琼脂糖凝胶电泳分析.结果经MspΙ酶切后,鲁道夫对盲囊线虫姊妹种与C .septentrionale表现出不同的条带;经NsiΙ酶切后,鲁道夫对盲囊线虫B和C .septentrionale结果一致,与鲁道夫对盲囊线虫A不同.用MspΙ可以鉴定出C .septentrionale ,用NsiΙ可以鉴定出鲁道夫对盲囊线虫A ,2个限制性内切酶合用可以将鲁道夫对盲囊线虫A、鲁道夫对盲囊线虫B以及C .septentrionale分别鉴定出来.根据鲁道夫对盲囊线虫A、鲁道夫对盲囊线虫B以及C .septentrionalerDNAITS 1基因序列建立的鲁道夫对盲囊线虫姊妹种PCR RFLP鉴别技术能够对鲁道夫对盲囊线虫姊妹种进行准确。 Using a pair of conserved primers, the first internal transcribed spacer (ITS-1) of ribosomal DNA (rDNA) of Contracaecum rudolphii complex and C. septentrionale were amplified by PCR. The purified amplicons were digested by Msp Ι and Nsi Ι selected according to the ITS-1 sequences of C. rudolphii A, C. rudolphii B and C. septentrionale, followed by agarose gel electrophoresis analyses. The banding profiles after digestion with Msp Ι were different between the C. rudolphii complex and C. septentrionale, and the profiles of C. rudolphii B digested by Nsi I was identical to that of C. septentrionale, but different from that of C. rudolphii A. In combination of using Msp Ι and Nsi I, C. rudolphii A, C. rudolphii B and C. septentrionale could be unequivocally differentiated. The PCR-RFLP approach was simple, specific and provided a useful molecular tool for the identification of the C. rudolphii A, C. rudolphii B and C. septentrionale.

关 键 词： 鲁道夫对盲囊线虫 姊妹种 第一内转录间隔区 鉴别技术

领　　域： [农业科学] [农业科学] [农业科学]