作　　者： ; ;

机构地区： 广东药学院基础学院

出　　处： 《卫生研究》 2005年第2期161-163,共3页

摘　　要： 目的　探讨锰接触对大鼠生后脑发育过程中时程因素和区域因素 (即时、空因素 )的影响作用。方法　将新生雄性SD大鼠随机分为染锰 1月龄组、2月龄组、3月龄组和各自对照组。染锰组自出生之日起供给 10g L氯化锰的溶液自由饮食 ,对照组供以自来水。 2 1日断奶后 ,取出母鼠 ,仔鼠继续供以上述溶液至处死之日止。各组动物于染锰结束后 ,采用胶质细胞纤维酸蛋白 (GFAP)免疫细胞化学染色与微机图象分析相结合技术 ,检测锰接触大鼠脑部不同时程和不同区域的胶质细胞纤维酸蛋白免疫反应情况及其反应阳性产物的平均相对密度。结果　 (1)染锰 1月龄组仔鼠 ,脑部的躯体感觉皮质和躯体运动皮质的GFAP阳性产物平均相对密度与对照组相比差异无显著性 ;而内侧隔核、斜角带、杏仁复合体、苍白球和无名质等锥体外系结构的GFAP阳性产物平均相对密度与对照组相比均显著升高 (P <0 . 0 0 1)。 (2 )染锰 2月龄组仔鼠 ,脑部的躯体感觉皮质和躯体运动皮质的GFAP阳性产物平均相对密度与对照组和 1月龄组相比 ,差异均无显著性 ;而锥体外系诸结构的GFAP阳性产物平均相对密度除显著高于对照组外 (P <0 .0 0 1) ,亦明显高于 1月龄组 (P<0 .0 1)。 (3)染锰 3月龄组仔鼠 ,脑部躯体感觉皮质、躯体运动皮质和锥体外系诸结构的GFAP阳性? Objective\ To investigate the effects of period and ar ea factors of manganese exposure on the postnatal development of brain in rats.Methods A combination of GFAP cytoimmunochemistry and imaging analysis was used to determine the morphological changes of glial cells and the average proportional densities of GFAP-immunochemistry positive products of dif ferent brain areas in different Mn-exposured period.Results (1 )in both one month's and two month's groups, no differences were found in the GF AP-immunochemistry extensities and the average proportional densities of GFAP r eaction positive products between the Mn-exposured group and the control group in the somatic motor and sensory cortexes, but in the expyramid nuclei or areas, that were increased significantly than the the control group.(2) In three month 's Mn-exposured groups, the GFAP-immunochemistry extensities and the average p roportional densities of GFAP reaction positive products in all related areas of three groups were previously increased than that of the control group. Conclusion In the interferential factors of mnganese exposure on the po stnatal brain development, both the Mn-exposure period and the areas were the i mportant ones.

关 键 词： 锰接触 脑发育 毒性影响 时程因素 区域因素

领　　域： [生物学] [生物学]