作　　者： ; ; ; ; ;

机构地区： 福建农林大学植物保护学院生物农药与化学生物学教育部重点实验室

出　　处： 《植物保护学报》 2004年第4期347-352,共6页

摘　　要： 辣椒体内的枯草芽孢杆菌BS-2菌株在白菜体内定殖、促生和防病作用表明:①用抗利福平标记BS-2r株浸种、浇灌土壤和涂抹叶片等方法接种,菌株均能进入白菜体内,并可在其全生育期内定殖;②菌液浸种24h后播种20天,其苗的鲜重比清水对照增加了91.20%～138.04%;③菌株对白菜炭疽病有较好的防治效果,但对不同时间接种病原菌的防治效果有所不同,以叶表面喷雾接种菌液后,立即接种病原菌的防效最好,其第3天和第6天的防效分别达95.12%和46.71%;而对接种菌液之前已接种病原菌的处理无防病效果.④菌株胞外分泌物对炭疽病菌菌丝生长、分生孢子产生与萌发均有很强的抑制作用.由此可见,来自辣椒体内的枯草芽孢杆菌BS-2 菌株不仅可在白菜体内定殖传导,而且对白菜有明显的促生作用,同时对炭疽病还有良好的防治作用. The functions of endophytic bacterium BS-2(Bacillus subtilis endophyticus)isolatedfrom Capsicum annuum were investigated.The results showed that ① BS-2 which mutanted byantagonism against the rifampicin was able to colonize,propagate and move in cabbage plantsinoculated with seed dipping,root watering and leaf daubing;②cabbage seeds were dippedfirst for 24h then sowed in the soil and the fresh weight of cabbage plants was increased by91.20%-138.04% in 20d after the seeds sowed;③if the plants were inoculated with an-thracnose pathogen,Colletotrichum higginsianum,immediately after spraying BS-2 suspension,the best control effect was obtained,the effects in the third and sixth day were 95.12% and46.71% respectively;if the plants were inoculated with the pathogen 1d before spraying BS-2suspension,the control effect was not found;④the antibiotics secreted by BS-2 showed stronginhibitory activity against the pathogen's mycelial growth and conidia formation and germina-tion.All these suggested that the BS-2 strain could colonize in the cabbage plants,promotegrowth of cabbage,and control its anthracnose disease.

关 键 词： 白菜 定殖 辣椒 接种 菌株 浸种 促生 枯草芽孢杆菌 内生菌 萌发

领　　域： [农业科学] [农业科学] [农业科学] [农业科学]