作　　者： ; ; ; ;

机构地区： 湛江海洋大学

出　　处： 《中国水产科学》 2005年第2期201-206,共6页

摘　　要： 采用垂直板聚丙烯酰胺梯度凝胶电泳对企鹅珍珠贝[Pteriapenguin(R ding)]的消化盲囊、鳃、外套膜、足部肌肉和闭壳肌肌肉5种组织中的过氧化物歧化酶(SOD)、酯酶(EST)、苹果酸脱氢酶(MDH)、苹果酸酶(ME)、乳酸脱氢酶(LDH)和葡萄糖-6-磷酸脱氢酶(G6PDH)同工酶的表达变化进行比较研究。结果表明,企鹅珍珠贝组织内的这6种同工酶存在不同程度的组织特异性,其位点的表达与否和酶活性的强弱方面都表现出明显的组织差异。企鹅珍珠贝的SOD、EST同工酶在消化盲囊中有较高活性,而MDH、ME在闭壳肌中活性较高。研究中讨论了这些差异与组织生理功能的相关性。 The wing oyster (Pteria penguin Rding)is a kind of large-sized and commercially important bivalve species,distributing along the coast of Hainan Island,Leizhou Peninsula and other maritime regions in Southern China Sea.The farming of and trial pearl cultivating from this species was successfully developed in recent years.There was very little information about its biochemical hereditary background before.The differentiation of five isozymes (SOD,EST,MDH,ME,LDH and G6PDH) from different adult tissues (digestive diverticula,gill,mantle,foot,adductor) of P.penguin were analyzed by vertical polyacrylamide gradient gel electrophoresis.The tissue specificity of these isozymes is very obvious in the results;the expression of enzyme gene loci and the activity of isozyme varied with different tissues,for example,the zymograms of SOD and EST from mantle tissue and MDH from adductor muscle distinctly contrasted to those from other tissues.The tissue-specific expressions of isozyme exhibited as follows.1) Some isozymes expressed restrictedly to certain tissues while no expression or displaying very low activity in other tissues,for example,the bands of Sod-3 were distinct only in mantle tissue,the same as Est-6 which only expressed in foot and adductor.2)The activities of certain isozymes obviously varied in different tissues,for example,Sod-2 and Est-4 had more strong expression in digestive diverticula than in other tissues.The physiological significance of differentiation of isozyme phenotypes was discussed in relation to the specific physiological functions of different tissues.It was supposed that the high activities of SOD (EST) in digestive diverticula and MDH (ME) in adductor muscle might meet the different physiological requirement of the two tissues respectively,that meant very high activities of EST and SOD in digestive diverticula were adapted for its physiological functions,digestion of food and protection from superoxide.

关 键 词： 企鹅珍珠贝 同工酶 组织特异性 基因位点

领　　域： [生物学]