作　　者： ; ;

机构地区： 中国科学院上海生命科学研究院生物化学研究所

出　　处： 《生物化学与生物物理学报》 1994年第5期565-569,共5页

摘　　要： 红花菜豆凝集素与莫寡糖部分分子间的聚合施炜星，孙册（中国科学院上海生物化学研究所，上海，２０００３１）关键词红花菜豆凝集素；寡糖；分子间聚合凝集素是一类与糖结合并凝集细胞或沉淀含糖大分子的蛋白质或糖蛋白。凝集素均由亚基组成，通常一个亚基具有一个结合糖... The purified lectin from Phaseolus coccineus var.rubronanus(PCI) showed a major protein band and a thin, minor one with less mobility by PAGE,but only one band by SDS-PAGE with or without meroaptoethanol. This thin band disappeared when Gly-HCI buffer containing mannan was used for eluting PCL from the thyro globulin-Sepharose affiniry column.However) the use of mannose or arabinogalaotan did not affect this thin band.Also the PAGE pattern of PCL when further purified on gel filtration or ion exchange chromatographie column was not changed. Both the H-oligosaeeharides(H-Man8 XylGlcNAc2(Ⅰ) and 3H-Man6Xy1Gl1cNAc2Fuc(Ⅱ)) prepared from PCL bound to immobilized PCL column and could be eluded from the column by phosphate buder containing mannan,thus demontwating the formation of intermoleeular polymerization through non-covalent binding of the oligosaccharide of one protein molecule to the carbohydratesbinding site of another molecule of lectin.

关 键 词： 红花菜豆 凝集素 寡糖 分子间聚合

领　　域： [生物学]