作　　者： ; ; ; ; (铁锋）;

机构地区： 北京大学基础医学院生物化学与分子生物学系

出　　处： 《生物化学与生物物理进展》 1994年第5期447-450,共4页

摘　　要： 将二价铜离子螯合在ＣｈｅｌａｔｉｎｇＳｅｐｈａｒｏｓｅＦａｓｔＦｌｏｗ凝胶上制成亲和层析柱，锌诱导兔肝和镉诱导小鼠肝经匀浆、乙醇处理后上柱，用ｐＨ４．０的醋酸盐缓冲液平衡，再用ｐＨ５．２不同浓度的醋酸盐缓冲液分别洗脱，可得两个金属硫蛋白（ＭＴ）洗脱峰，经确定先后为ＭＴ－２和ＭＴ－１．分离方法比传统的凝胶过滤－离子交换法简单、省时，适于实验室规模分离纯化． (China), An affinity chromatography column for isola-tion and purification of metallothionein (MT)was prepared with Chelating Sepharose FastFlow gel bound with bivalent copper. Zinc-in-duced rabbit liver. or cadmium-induced mouseliver was homogenized and precipitated withethanol. The sample was applied on the col-umn and equilibrated with pH4. 0 acetic acidbuffer. Then pH5. 2 of different concentrationacetic acid buffers were used for elution ofMT . Two eluted peaks were obtained andidentified as MT-2 and MT-1. Comparingwith the traditional method -gel filtrationand ion exchange chromatography. thismethod is simple and time-saving in laborato-ry-scale.

关 键 词： 金属硫蛋白 金属螯合 分离 提纯

领　　域： [生物学]