作　　者： ; ;

机构地区： 华南师范大学生命科学学院生物科学系

出　　处： 《华南师范大学学报（自然科学版）》 1994年第2期46-50,共5页

摘　　要： 本实验获得了抗链霉素突变株ＭＸＤＩ６Ｓ＿ｍ￣Ｒ，此菌株既有稳定的抗药性又保持良好的固氮能力．菌株间竞争结瘤表明ＭＸＤＩ６Ｓ＿ｍ￣Ｒ竞争能力量强，并有双侵染出现．ＭＸＤＩ６Ｓ＿ｍ￣Ｒ与土著根瘤菌竞争时，竞占瘤率达９０％．生长在ＴＹ培养基上的ＭＸＤＩ６Ｋ＿ｍ￣Ｒ与生长在ＹＥＭ培养基上的ＭＸＤＩ６Ｓ＿ｍ￣Ｒ竞争时ＭＸＤＩ６Ｋ＿ｍ￣Ｒ占瘤率为零． Strain MXDI6S_m￣Ragainst streptomycin was obtained in the present experiment.It kept antibioticresistance and N_2 fixation ability for generations.Competitive nodulation between different strainsshowed that strain MXDI6S_m￣R was a superior competitor,and double infection was discovered.Strain MXDI6S_m￣R was present in 90%of the nodules in competitive nedulation between strainMXDI6S_m￣R and native strains.Strain MXDI6K_m￣R was not presentin nedules in competitivenedulation between strain MXDI6K_m￣R growing in TY medium and strain MXDI6S_m￣R growing inYEM medium.

关 键 词： 根瘤菌 竞争结瘤 抗菌素抗性 双侵染 绿豆

领　　域： [生物学] [农业科学] [农业科学]