作　　者： ; (肖溆林）;

机构地区： 复旦大学生命科学学院微生物学与微生物工程系

出　　处： 《复旦学报（自然科学版）》 1994年第2期148-156,共9页

摘　　要： 用细胞临界生长密度法对棉铃虫蛹卵巢细胞系ＳＦＥ—ＨＡ—８２１２进行克隆化，获得８株克隆细胞系．各株克隆细胞系各自的形态较为均一，但相互间在形态、生长特性、对病毒受纳性等方面有很大差别，其中一株ＣＳ细胞对棉铃虫单粒包埋型核型多角体病毒（ＨａＳＮＰＶ）的受纳性明显高于ＳＦＥ—ＨＡ—８２１２细胞，形成多角体的细胞比率、多角体的产量、游离病毒粒子的产量及多角体蛋白的产量均有提高，是研究和应用ＨａＳＮＰＶ的有效系统． he critical cell growth density method was used in cloning the cotton bollworm Heliothis armigera cell line, SFE-HA-8212. Growth of the cell was found to be closely related to cell density. When the latter was too low, little growth of cells were observed. The cell density that permitted only a few of them to grow was called the critical cell growth density. These growing cells were traced till the establishment of clones. Eight clones were obtained by this means. Cells of one clone were morphologically homologous, but distinct from those of other clones in morphology, growth characters and susceptibility to nuclear polyhedrosis virus infection. Only one clone C8 was significantly more susceptible to HaSNPV infection than the parent cell line. The clone had a larger percentage of cells forming polyhedra, and larger yields of polyhedra, free vinous, and polyhedrin. C8 cell line could be a promising in vitro system for studying HaSNPV replication, and for biotechnical application as well.

关 键 词： 细胞培养 细胞克隆 棉铃虫

领　　域： [生物学]