作　　者： ; (徐浩大）;

机构地区： 北京大学基础医学院生物化学与分子生物学系

出　　处： 《生物化学杂志》 1989年第3期193-198,共6页

摘　　要： 以猪血清为材料,通过磷酸乙醇胺—琼脂糖亲和层析,Sepharose 4B柱层析和Sephacryl—S300凝胶过滤,获得了猪C—反应蛋白的结晶。猪C—反应蛋白可与肺炎球菌壁C多糖发生特异的沉淀反应,这种结合是依赖钙离子的。EDTA和一些磷脂代谢产物如磷酸胆碱,磷酸乙醇胺等,能抑制猪C—反应蛋白与C多糖的结合。在SDS—聚丙烯酰胺凝胶电泳及梯度聚丙烯酰胺凝胶电泳中,猪C—反应蛋白表现出与人C—反应蛋白相同的行为,亚基是一条分子量为23.5kD的肽链,全分子的表观分子量为150kD。猪C—反应蛋白与兔抗人C—反应的蛋白的抗血清能发生免疫交叉反应。 Porcine C-reactive protein (CRP) has been isolated and purified by affinity chromatography on PE-Sepharose, chromatography on Sepharose-4B and gel filtration on Sephacryl-S 300 column. Pure porcine CRP preparation could be obtained by the present method to provide material for further investigations and for crystallization.The porcine CRP can be precipitated by pneumococcal C-polysaccharide in the presence of Ca++ ion. The precipitation is inhibited by either EDTA or by the metabolic products of lecithin, such as a-glycerophosphorylcholine, CDP-choline,phospho-ryl-choline and phosphorylethanolamine.The electrophoretic behavior of porcine CRP in SDS-PAGE and gradient PAGE is the same as human CRP. The apparent molecular weight of porcine CRP is approximately 150 kD daltons, while its subunit has an apparent molecular weight of 23.5kD. Porcine CRP crossroads imm-unologically with rabbit antisera against human CRP.

关 键 词： 猪 反应蛋白 纯化 性质

领　　域： [生物学]