机构地区: 暨南大学生命科学技术学院生物工程学系
出 处: 《暨南大学学报(自然科学与医学版)》 1993年第1期67-72,共6页
摘 要: 利用大肠杆菌质粒pUC18、pTG206和金黄色葡萄球菌质粒pC194在体外构建3个嵌合质粒pCC10、pCCT7和pST16.3个新质粒均是大肠杆菌和枯草杆菌的穿梭质粒。它们在大肠杆菌中呈Ap^rCm^r型,在枯草杆菌中则为Cm^rAp^5型。其中pCCT7和pST16含有xylE基因,可作为启动子探测质粒。所有的新质粒都具有来自pUC18的多酶克隆位点,适合于在大肠杆菌和枯草杆菌中重组外源DNA以及比较它们的表达情况。 Three chimereic plasmids, pCC10 pCCT7 and pST16 were constructed from E.coli plasmids pUC18, pTG206 and Saureus plasmid pC194 by enzymatic manipulation. These new plasmids were all shuttle plasmids and can replicate in both E.coli and S.subtilis. As expected, they have shown a Cm^r Ap^5 phenotype in B.substilis and Cm^r Ap^r phenotype in E.coli. The new plasmids pCCT7 andpST16, containing xyIE structural gone, were suitable for cloning fragments of DNA that carry transeriptional promoter. All new plasmids contained polylinker from pUC18. They can be used to introduce the same gene into two bacterial hosts and compare the in expression.
领 域: [生物学]