作　　者： ; ; ; ; ;

机构地区： 华南理工大学食品与生物工程学院

出　　处： 《植物生理学通讯》 2004年第6期702-704,共3页

摘　　要： 胡桐CR2细胞悬浮培养过程中,苯丙氨酸解氨酶(PAL)活性上升后红厚壳素产量即增加。加入真菌诱导子后,PAL 活性与红厚壳素产量呈一定的正相关。以PAL抑制物降低PAL活性后,红厚壳素产量也降低;诱导物与抑制物同时加入, PAL活性与红厚壳素产量均界于诱导物处理与未处理之间。 Changes in phenylalanine ammonia-lyase (PAL) activity and inophyllums production were analysed, showing that PAL activity increased preceding the inophyllums production during the process of suspension culture. PAL activity changed after adding fungal elicitor, followed by changes of inophyllums production. Addition of the PAL inhibitor L-2-amono-3-phenylpropionic acid at 10 μmol·L-1 suppressed the induction of PLA activity and the corresponding inophyllums production. When the fungal elicitor was added with the inhibitor, the both PAL activity and inophyllums production were between those induced by fungal elicitor and control. There existed a significantly correlation between PAL activity and inophyllums yield in CR2 suspension cultured cell of Calophyllum inophyllum.

关 键 词： 产量 苯丙氨酸解氨酶 诱导物 酶活性 胡桐 活性 细胞悬浮培养 悬浮培养细胞 真菌诱导子 抑制物

领　　域： [生物学] [农业科学] [农业科学]