作　　者： ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《亚热带植物科学》 2004年第4期37-38,41,共3页

摘　　要： 一品红不同部位愈伤组织诱导能力存在差异,嫩茎>幼花序>嫩叶。愈伤组织的长势主要受生长素的影响,细胞分裂素对愈伤组织生长有促进作用;但在含 6-BA 和 NAA 的培养基中诱导出的愈伤组织,其胚性明显强于单独用 NAA 诱导出的愈伤组织。液体悬浮培养是一品红体细胞胚胎高频发生的中间步骤。不同浓度BA 对一品红体细胞胚的萌发率影响不大,萌发培养基中 KNO3 含量加倍可提高萌发率。 The callus induction varied in different parts of Euphorbia pulcherrima. The young stem was more effective than the young inflorescens, and the young leaf was less effective than the formers. The growth speed of callus depended mainly on auxin, and was promoted by cytokinin. Embryogenesis frequency of the callus induced on MS medium with 6-BA and NAA was higher than those induced on MS medium with only NAA. Suspension culture was regarded as a intermediate step to promote embryo production. The concentrations of 6-BA in the MS medium has no influence on the embryo germination frequency. Doubling the concentration of KNO3 in the medium promoted embryo germination.

关 键 词： 一品红 组织培养 体细胞 胚胎发生 植株再生

领　　域： [生物学] [农业科学] [农业科学]