作　　者： ; ; ; ; ; ;

机构地区： 华南农业大学资源环境学院环境科学与工程学系

出　　处： 《仲恺农业技术学院学报》 2004年第4期1-6,共6页

摘　　要： 采用PDA、OMA及苜蓿煎汁+Czapek培养基,对22种在自然寄主植物上缺少乙型分生孢子的拟茎点霉(Phomopsis)进行了培养.结果表明,16个种在PDA或苜蓿煎汁+Czapek培养基上(25℃,14h/d光照)培养10～20d后能产生乙型分生孢子,除花烛拟茎点霉(Phomopsisanthurii)、腊梅拟茎点霉(P.chimonanthii)和紫薇拟茎点霉(P.lagerstroemiae)的部分乙型分生孢子有变态外,其余种的孢子形态与该属真菌典型的乙型分生孢子基本一致;坚木拟茎点霉(P.dysoxyli)可产生与甲、乙型分生孢子形态完全不同的丙型分生孢子.苜蓿煎汁+Czapek培养基为产生乙型分生孢子的最佳培养基. Twenty two species of Phomopsis that lacked β-conidia on the natural host plants were cultured on PDA, OMA and alfalfa extract plus Czapek respectively. The results indicated that 14 species could produce β-conidia on PDA or alfalfa extract plus Czapek after being incubated in 7～15 d at 25 ℃, 14 h/d illumination. In pure culture most species produced typical β-conidia, which were almost the same morphologically with those from other Phomopsis species. However P. anthurii, P. chimonanthii and P. lagerstroemiae formed much wider and shorter β-conidia. P. dysoxyli could produce γ-conidia, which is quite different from α- and β-conidia. The study showed that alfalfa extract plus Czapek was the best media for producing β-conidia, and the transition of OMA could help to produce β-conidia.

关 键 词： 拟茎点霉 分生孢子 培养基

领　　域： [生物学]