作　　者： ; ; ; (王革）; (李天飞）;

机构地区： 云南省烟草科学研究所

出　　处： 《烟草科技》 2004年第11期43-45,共3页

摘　　要： 为了解伴孢晶体的类型,采用液体双相分离法分离提纯苏云金杆菌Bt33、Bt53和BtHB伴孢晶体,其分离纯度分别达到99 9%、99 9%和99 8%。扫描电镜和透射电镜观察发现,3个菌株均有菱形、长椭圆形和不规则形晶体,个别有方形,少部分长椭圆形晶体为不标准长椭圆形,带有"拖尾"或突起。不同菌株在晶体形状和数量组成上有所不同,Bt33菌株菱形晶体为75%、长方形与长椭圆形晶体为22%～23%,其余为不规则形;Bt53菌株,长椭圆形、菱形和不规则形晶体各为90%、5%和5%;BtHB菌株,其菱形、长椭圆形和方形晶体分别为30%、64%～65%和5%,其余为不规则形。上述晶体大小不均一。 In order to understand the morphological characteristics of parasporal crystals, the parasporal crystals of 3 Bacillus thuringiensis strains: Bt33?Bt53 and BtHB were extracted with liquid diphasic separation, the separation purity was Bt33 99.9%, Bt53 99.9%, BtHB 99.8%, respectively. Under scanning electron microscope and transmission electron microscope, the findings were that all of 3 strains had rhombic, long oval or irregular crystals, several strains contained square crystals, a few long oval crystals had 'trail' or protuberance; different strains had different crystal numbers and shapes. The proportion of rhombic crystal in Bt33 was 75%, that of rectangular and oval crystal was 22%-23%, and the rest was in irregular form. For Bt53, 90% was long oval, 5% was rhombic, and the other 5% was irregular form. For BtHB, 30% was rhombus crystal, 64%-65% was long oval, and 5% was square. The crystal size was differed.

关 键 词： 苏云金杆菌 伴孢晶体 形态特征 提纯

领　　域： [农业科学] [农业科学]