作　　者： ; ; ; ; ; ;

机构地区： 中国人民解放军第一军医大学第二附属医院

出　　处： 《解剖学报》 2004年第6期664-667,共4页

摘　　要： 目的　标记小鼠Y染色体RNA探针 ,检验该探针在检测外周血涂片和脑组织切片Y染色体阳性细胞的效率。　方法　以小鼠Y染色体重复序列pY35 3/BcDNA为模板标记RNA探针。以雌性小鼠为对照 ,用原位杂交法检测小鼠外周血涂片Y染色体阳性细胞 ;用荧光原位杂交法检测雄性小鼠脑组织切片Y染色体阳性细胞。　结果　Y染色体原位杂交法在外周血涂片Y染色体阳性的检测灵敏度和特异度均为 1 0 0 %,在脑切片检测Y染色体阳性细胞的灵敏度和特异度分别为 85 6 7%和 1 0 0 %。 Objective The RNA probe from the mouse Y chromosome sequence was labeled and used to test its efficiency for detecting Y chromosome positive cells in peripheral blood smear and brain tissue sections in mice. Methods The RNA probe was labeled from pY353/B, a repeat sequence of mouse Y chromosome. Compared with the female mice, the peripheral blood smear and brain tissue sections of the male mice were detected with in situ hybridization(ISH) and fluorescence in situ hybridization (FISH). Results The sensitivity and specificity for detecting of Y chromosome positive cells in peripheral blood smear is 100%, and the sensitivity and specificity for detecting of Y chromosome positive cells in brain tissue is 85.67% and 100% respectively.Conclusion The high sensitivity and specificity for detecting of Y chromosome positive cells in peripheral blood smear and brain tissue sections of mice is proved by Y chromosome ISH and FISH.;

关 键 词： 染色体 原位杂交 荧光原位杂交 小鼠

领　　域： [生物学] [生物学]