作　　者： ; ; ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《园艺学报》 2004年第5期688-690,共3页

摘　　要： 利用墨兰与大花蕙兰进行种间杂交 ,成功获得了 5个组合的杂种原球茎和植株。墨兰×大花蕙兰及大花蕙兰×墨兰 ,均以原球茎方式萌发。以墨兰×大花蕙兰的杂种原球茎为材料 ,通过L9(34)正交设计研究了NH4NO3 、KH2 PO4、 6 BA和NAA等因素对杂种原球茎增殖的影响 ,发现NAA对原球茎的增殖影响不大 ,NH4NO3 16 5 0mg·L-1(MS正常浓度水平 ) ,提高KH2 PO4浓度 (170～ 85 0mg·L-1)和 6 BA浓度 (1～ 10mg·L-1)有利于原球茎的增殖。 9个培养基中以MS +NH4NO3 16 5 0mg·L-1+KH2 PO4340mg·L-1+6 BA 10mg·L-1效果最好。 Hybrid plants were obtained from 5 crosses between Cymbidium sinense and Cymbidium hybridium.The genotype of germination by protocorm like-body(PLB)of C.hybridium is controlled by dominant gene(s).Because all of these hybrid embryos,whatever C. sinense×C.hybridium or C.hybridium×C.sinense,are germinated by PLB,which is the same as C.hybridium.Four factors of NH_4NO_3、KH_2PO_4、6-BA and NAA with three different concentration(L_9)were designed to study the effects on induction and propagation of PLB of C.‘Jin Zui ’×C.‘Forgotten Fruits’.Analysis of variance shows: The different concentration among the factors which are NH_4NO_3、KH_2PO_4 and 6-BA influences the induction and propagation of PLB directly, There is no difference with different concentration of NAA. NH_4NO_3 1650 mg·L -1(MS normal levels), higher concentration of KH_2PO_4(170-850 mg·L -1)and BA densities(1-10 mg·L -1)was favorable to propagation of PLB. There are extremely remarkable differences among 9 media, the best is C_5 medium with NH_4NO_3 1650 mg·L -1,KH_2PO_4 340 mg·L -1and 6-BA 10 mg·L -1.

关 键 词： 墨兰 大花蕙兰 种间杂种 原球茎 诱导增殖

领　　域： [农业科学] [农业科学]