作　　者： ; ; ;

机构地区： 哈尔滨师范大学生命科学与技术学院生物科学系

出　　处： 《动物学报》 2004年第5期817-822,共6页

摘　　要： 小球藻绿钟虫 (VorticellachlorellataStiller ,194 0 )采自哈尔滨附近水域。本文在活体观察基础上 ,利用蛋白银染色技术对其形态学进行了研究 ,给出了该种染色后的特征。小球藻绿钟虫因其细胞内充满共生小球藻呈鲜绿色而得名。该种自然伸展时呈稳定的矮钟状 ,体长 5 3- 6 3× 4 1- 6 2 μm ;大核“J”型 ,两端明显盘绕 ,纵贯于体内。细胞表面具横纹 ,蛋白银制片后可见从口围唇到反口纤毛环之间有 33- 36条纹 ,从反口纤毛环到帚胚有条纹 15 - 18条 ;第三咽膜 (P3)由三列毛基列组成 ,最内侧一列长度仅是另外两列的一半 ,并显著分离 ;口围盘纤维斜向并呈轮辐式放射状排列 ,纤维末端分叉。通过对本文描述的绿色钟虫—小球藻绿钟虫的研究 ,发现对绿色钟虫的描述与归属问题仍存在一些混淆。 The morphology, infraciliature and myonemic system of Vorticella chlorellata (Stiller, 1940), collected from a eutrophic freshwater pond near Harbin, China, was studied using living and protargol-impregnated specimens. No recent data derived from silver impregnation on this species are available, and an emended diagnosis and description of it are provided. Freshwater Vorticella, zooid in vivo 53-63 × 41-62 μm, stout in shape; cell conspicuously green-colored with symbiotic green algae; one macronucleus “J”-shape, but rather curved in both end. The number of transverse striations from peristomial area to aboral ciliary wreath was 33-36, from aboral ciliary wreath (ACW) to scopula, 15-18. The adstomal end of peniculus 3 is rather dispersed. Peristomial disc fibers are conspicuously oblique radiate assignment, with dichotomous end in each fiber. Through the study of characteristics of the green Vorticella species, we found there still existed some confusions and believed it was wrong to identify V.chlorellata with Vorticella chlorostigma in many descriptions. The green species redescribed in this paper was V.chlorellata, but not V.chlorostigma.

关 键 词： 纤毛虫 形态学 蛋白 银染色 细胞表面 体内 细胞内 小球藻 大核 活体观察

领　　域： [生物学] [生物学]