作　　者： ; ; ;

机构地区： 佛山科学技术学院

出　　处： 《广西植物》 2004年第5期463-465,共3页

摘　　要： 采用NBT光化还原法,分别研究了不同缓冲溶液、pH值、温度及抑制剂等因素对南瓜种子SOD活性的影响,并对其类型进行了鉴定,结果表明:南瓜种子萌芽过程中SOD的合成以Mn-SOD为主,其热稳定性很高,在酸性条件下活性完全丧失,在pH8.0 Na2HPO4-NaH2PO4缓冲溶液下酶活性最高;酶活性容易被β-巯基乙醇抑制,对脲及DMSO有一定的耐受性。 The activities of superoxide dismutase (SOD) of Cucurbita sp. seeds were studied with the method of the photochemical reduction of nitrio blue terazolium (NBT) in different cushioning liquer, pH, temperature and inhibitor. The form of SOD were also studied. The results showed that: Mn-SOD was composed mainly in the seed process and the heat stability was very high. The activity was the highest in pH8. 0 of cushioning liquer of Na2HPO4-NaH2PO4 and losed completely in acid condition. The activity was inhibited easily by β-mercaptoethanol and able to bear urea and DMSO in a certain extent.

关 键 词： 南瓜 种子萌芽 酶活性 温度 活性 超氧化物歧化酶 鉴定 巯基乙醇 耐受性

领　　域： [生物学] [农业科学] [农业科学]