作　　者： ; ; ; ; ;

机构地区： 广州出入境检验检疫局

出　　处： 《中国动物检疫》 2004年第11期23-26,共4页

摘　　要： 采用《中华人民共和国出入境检验检疫行业标准》"副结核病补体结合试验操作规程"方法(简称常量法)和微量补体结合试验(简称微量法)检测3677头进口牛羊的副结核病抗体并进行比较。结果表明,常量法和微量法的溶血素效价测定值相同,补体效价测定值近似,阴性血清对照、抗原对照、补体对照、红细胞对照、血清抗补体结合对照结果均相同,阳性对照血清效价微量法比常量法提高1个滴度,被检血清的阳性检出率微量法高于常量法。比较37℃水浴及37℃恒温培养箱环境下的微量法补体结合试验结果,溶血素效价、补体效价、各项对照试验结果完全相同,被检血清在37℃水浴反应更加充分。比较用变态试验和补体结合试验检验3677头牛羊副结核病的结果、以及用补体结合试验、变态反应和酶联免疫吸附试验方法同时检测2024头牛的结果,发现它们的相关性很小。 3 677 cattle and sheep were examined respectively by microcomplement fixation test (microtitre method for short) and 'Procedure of complement fixation test for paratuberculosis' from《Standards of Entry - Exit Inspection and Quarantine of P. C.R.》(constant method for short) for paratuberculosis and the titers of the paratuberculosis antibody were compared. It was shown that the titers of hemolysin, complement, negative conlrol serum, antigen control, complemenl control, haemolytic system control and serum anti - complement conlrol were the same by the two methods. But the titer of positive conlrol serum of microtiter method was 1 titer higher than that of the constant method, and the positive rate of the examined sera by the microtiter method was higher than that by the constant method. In addition, serum inactivation in 37℃ water bath was better than that in 37℃ incubator. 2 024 cattle were examined respectively by microcomplement fixation test, delayed hypersensitivity test and ELISA, suggesting that the results by the three different tests had little correlation.

关 键 词： 牛 羊 副结核病 微量补体结合试验 抗体检测

领　　域： [农业科学] [农业科学] [农业科学] [农业科学] [农业科学] [农业科学]