作　　者： ; ; ; ;

机构地区： 华南农业大学动物科学学院

出　　处： 《华南农业大学学报》 2004年第4期78-81,共4页

摘　　要： 将IBDV超强毒株HK46的结构蛋白vp2基因的cDNA片段插入表达质粒pSOC的EcoRI位点,获得重组质粒pSOC VP2.用pSOC VP2转化E.coliBL21(DE3),获得表达VP2蛋白的工程菌BL SOC VP2.在1μg/mLIPTG诱导之下,BL SOC VP2表达了融合蛋白SOC VP2,其相对分子质量为49000.SDS PAGE检测表明,融合蛋白SOC VP2的最大表达量为细菌总量的14 35%.ELISA分析表明,大肠杆菌表达的SOC VP2能与IBDV阳性血清发生特异性反应. The cDNA fragments of gene vp2 of very virulent infectious bursal disease virus strain HK46 were cloned into the EcoRI site of expression plasmid pSOC to obtain recombinant plasmid pSOC-VP2. Then the recombinant plasmid pSOC-VP2 was conducted into the competent cell of BL21 (DE3) by Ca^(2+) to obtain the engineering bacterial strains BL-SOC-VP2. By addition of IPTG at end concentration of 1 μg/mL, BL-SOC-VP2 expressed SOC-VP2 fusion protein with relative molecular mass (49 000). It was showed by density-metry scanning on SDS-PAGE gel that the largest amount of SOC-VP2 fusion is 14.35% of total bacterial protein. It was demonstrated also by ELISA that bacteria expressed SOC-VP2 which specifically reacted with antisera against IBDV.

关 键 词： 传染性囊病 病毒结构 蛋白 融合表达 分子质量

领　　域： [农业科学] [农业科学] [农业科学]