作　　者： ; ; ; ; ;

机构地区： 中国海洋大学水产学院海水养殖教育部重点实验室

出　　处： 《水生生物学报》 2004年第5期496-503,共8页

摘　　要： 利用双因素实验设计研究了在饲料中添加维生素E(VE) (0 ,5 0IU/kg)和硒 (Se) (0 ,0 2 ,0 6 ,1 5mg/kg)对皱纹盘鲍 (HaliotisdiscushannaiIno)血清中过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD)、依赖硒的谷胱甘肽过氧化物酶(GPX)、谷胱甘肽还原酶 (GR)、谷胱甘肽转移酶 (GST)这 5种抗氧化酶活力的影响。结果表明 :Se对皱纹盘鲍血清 5种抗氧化酶活力都有显著影响 (P <0 0 5 ) ,而VE仅对GPX和GR的活力有显著影响 (P <0 0 5 )。VE和Se对CAT、GRX和GR的活力的影响具有显著的交互作用。GPX/SOD、GR/GPX、CAT/SOD这 3个抗氧化的重要指标都表明 ,当饲料中含有 4 5 0IU/kg的VE时 ,添加 0 6mg/kg的硒能使皱纹盘鲍血清中的抗氧化物酶系统总体达到相对平衡 ,从而能有效地抵抗氧化损害。 When attacked by microbes,the immune system of the host can be activated,which induces many changes. One of them is that the hemocytes can produce reactive oxygen intermediate(ROIs)to kill the microbes. On the other hand,anti-oxidant system is necessary to maintain an appropriate amount of ROIs,and keep a good balance between killing microbes and protecting itself from hurt by superfluous ROls. Vitamin E(VE)and selenium(Se)are two important components of the anti-oxidant system,and can eliminate the superfluous ROIs directly or indirectly. In the present study,a two-factorial experiment was designed to evaluate the effects of dietary Se and VE on antioxidant enzyme activities,which can reflect the oxidant status of the organisms,abalone Haliotis discus hannai Ino. Totally eight semi-purified diets were formulated to provide graded levels of Se(0,0.2,0.6,1.5mg/kg)and VE(0,50IU/kg). Abalone of similar size(6—7cm)were distributed randomly into buckets with re-circulating sea water. Eight different artificial diets and one control(fresh kelp,Laminaria japonica)were tested. Each treatment had three replicates. The feeding experiments lasted for 120 days. The culture conditions during this period were as follows:12 hr illumination per day,water temperature 15.6—20.5℃,salinity 31—34,dissolved oxygen 7.2mg/L,pH 7.5—7.9. After the feeding experiments,the animals were sacrificed and hemolymph was withdrawn,and centrifuged at 3000 r/min for 5 min at 4℃. The supernatant was kept in -70℃ for further use. The activities of catalase(CAT),superoxide dismutase(SOD),glutathione peroxidase(GPX),glutathione reductase(GR),glutathion-S-transferase(GST)in serum were assayed. The results showed that dietary Se had more efficient effects on antioxidant enzyme activities than VE. Dietary Se had significant effects on all five antioxidant enzyme activities(P<0.05),while VE significantly influenced the activities of only GPX and GR(P<0.05). There were significant interactions between Se and VE on the activities of CAT,GPX a

关 键 词： 硒 维生素 皱纹盘鲍 血清 抗氧化酶

领　　域： [农业科学] [农业科学]