机构地区: 中山大学中山医学院法医学系
出 处: 《人类学学报》 2004年第3期239-247,共9页
摘 要: 揭示云南白族 136例无关男性个体Y染色体小卫星DYF15 5S1基因座多态性。用已建立的Amp FLP和MVR PCR方法分析DYF15 5S1基因座长度多态性、5′端多态性、3′端多态性。DYF15 5S1基因座具有高度多态性 ,基因多样性 (h)达 0 9996。研究表明将Amp FLP和荧光MVR PCR结合起来更能充分揭示DYF15 5S1基因座多态性 ,可为遗传学、人类学及法医学的研究提供有效的工具和基础资料。 This study is to reveal the polymorphism of DYF155S1 locus, Y-specific minisatellite, among 136 cases unrelated male in Bai population of Yunnan Province. The analytical methods include Amp-FLP(Amplified fragment length polymorphism) and MVR-PCR(minisatellite variant repeat by PCR)with type 1-specific primer at 5′ end and type 4-specific primer at 3′ end. It is found that DYF155S1 locus possesses a high polymorphism and its gene diversity (h) reaches 0.9996. These results indicated that Amp-FLP in combination with MVR-PCR may reveal fully the polymorphism of DYF155S1 locus and provide a useful tools and more data for the field of human genetics, anthropology and forensic medicine.