作　　者： ; ; ; ; ; ; ; ; ; ; ; ; ; (郝卫芳）;

机构地区： 山西农业大学动物科技学院

出　　处： 《畜牧兽医学报》 2015年第6期1018-1025,共8页

摘　　要： 研究鸡源大肠杆菌耐药性及中药对大肠杆菌耐药性消除作用。选择22种抗生素采用Kirby-Bauer法对山西省部分地区分离的20株致病性鸡源大肠杆菌进行耐药性检测,对分离菌株进行质粒分析和耐药质粒转化试验,并分析多种中药对大肠杆菌耐药性的消除作用。结果显示,20株试验菌对测试抗生素均存在不同程度的耐药性,其中5耐及5耐以上的菌株占分离菌株的90%,试验菌对青霉素耐药率最高(90%),其次为恩诺沙星(85%),而对黏杆菌素和呋喃妥因的耐药性最低;分离出12种质粒谱型,同一地区、同一鸡场菌株的质粒图谱相同或相似,不同地区菌株的质粒图谱不同,仅有部分相同或相近的流行质粒共存;对其中5株菌株进行耐药质粒的转化试验,发现同一质粒可编码1个至数个耐药性基因,不同质粒可以携带相同的耐药性基因;中药单剂或合剂对大肠杆菌的耐药性有一定的消除作用,其中黄芩的消除效果最好,中药处理后部分菌株质粒图谱无变化,只有黄连和双黄连造成2条质粒带丢失;对中药提取物作用后的消除子进行药敏试验,结果发现大肠杆菌对环丙沙星等多种抗生素恢复了敏感性。研究结果提示联合使用中药治疗鸡源大肠杆菌病有显著效果。 The aim of this study was to study the drug resistance of chicken isolated Escherichia coliand role of traditional chinese medicines in the drug resistance elimination.Twenty kinds of E.coli isolated from chicken around Shanxi province were tested to determine the resistant rate to22 kinds of antibiotic by Kirby-Bauer way,the plasmid analysis and transformation test were conducted.Based on the resistance test results to antibiotic,we also evaluated the elimination effectsof several traditional Chinese medicine(TCM)extracts on the isolated chicken E.coli.The results showed that the percent of E.coli resistant to above 5kinds of antibiotics reached 90%.The isolated E.coli exhibited the strongest resistant rate to Penicillin G(90%),followed by Enrofloxacin(85%),while exhibited the highest sensitivity to Colimycin and Furadantin.According to the plasmid analysis results,12 kinds of plasmid patterns in total were found.Plasmids collected from the near farms with each other were likely to have the similar patterns,while different types among diverse areas.The transformation test results showed that one plasmid could encode single or several resistance genes,and different plasmids could have same resistance gene as well.TCM extracts,especially Scutellaria baicalensis,had significant elimination function to the drug resistant E.coli.Majority of the TCM treated plasmids maintain the same patterns,while two plasmids change their profiles after treating with coptis chinensis and shuanghuanglian,losing two DNA bind respectively.After all,TCM extract treatment recovered the susceptibility of chicken E.coli to drugs,which support the permitted use of TCM on the chicken E.coli disease control.

关 键 词： 鸡 大肠杆菌 耐药 质粒 中药

领　　域： [农业科学] [农业科学] [农业科学]