作　　者： ; ; ; ; ; ; ; ;

机构地区： 东北农业大学动物医学学院

出　　处： 《畜牧兽医学报》 2014年第1期160-164,共5页

摘　　要： 为研究小型猪专用复合麻醉剂(XFM)对大鼠中枢神经系统c-myc mRNA和iNOSmRNA转录的影响,将30只大鼠随机分为对照组(C组)和麻醉剂组(M组),M组又根据时间分为4个亚组:M1组(注射XFM后大鼠翻正反射消失即刻)、M_2组(注射XFM后大鼠翻正反射消失后1 h)、M_2组(注射XFM后大鼠翻正反射恢复即刻)和M_4组(注射XFM后大鼠翻正反射恢复后1 h),各组大鼠到达预定的时间点后分别采取脑组织,应用实时荧光定量PCR技术检测中枢神经系统中c-myc mRNA和iNOS mRNA转录量。结果显示,大鼠在应用XFM后,大脑皮层和丘脑c-myc mRNA的相对转录量在麻醉期间受到明显的抑制,与对照组相比差异极显著(P<0.01),而小脑、海马和脑干c-myc mRNA的相对转录量在麻醉期间明显增加,与对照组相比差异极显著(P<0.01);所有脑区iNOS mRNA的相对转录量在麻醉期间受到明显的抑制,与对照组相比差异极显著(P<0.01)。结果表明XFM能够影响中枢神经系统中c-myc mRNA和iNOS mRNA的转录,这可能是其产生麻醉作用的机理之一。 In order to investigate the transcription of c-myc mRNA and iNOS mRNA in central nervous system in rats anesthetized with the Combined Anaesthetic for Miniature Pigs(named as XFM),30 rats were divided randomly into two groups:control group and XFM group.XFM group consisted of four subgroups:M_1 subgroup(rats received XFM intraperitoneally and then waiting the disappearance of right reflection);M_2 subgroup(rats received XFM intraperitoneally and then waiting for 1 h after the disappearance of right reflection);M_3 subgroup(rats received XFM intraperitoneally and then waiting the recovery of right reflection) and M_4 subgroup(rats received XFM intraperitoneally and then waiting for 1 h after the recovery of right reflection).The brain tissues were taken when reaching each time point.The transcription of c-myc mRNA and iNOS mRNA in central nervous system was detected with real time PCR.The results show that the transcription of c-myc mRNA in cerebral cortex and thalamus decreased significantly after receiving XFM(P<0.01);The transcription of c-myc mRNA in cerebellum,hippocampus and brain stem increased significantly(P<0.01).The transcription of iNOS mRNA in all brain regions decreased significantly after receiving XFM(P<0.01).These results indicated that XFM could affect the transcription of c-myc mRNA and iNOS mRNA in central nervous system.This might be one of the anaesthetic mechanisms for XFM.

关 键 词： 小型猪专用复合麻醉剂 中枢神经系统

领　　域： [农业科学] [农业科学] [农业科学]