作　　者： ; ; ; ; ; ;

机构地区： 河北农业大学

出　　处： 《畜牧兽医学报》 2014年第1期129-135,共7页

摘　　要： 为研究清道夫受体在树突状细胞提呈口蹄疫病毒VP-VP4融合蛋白中的作用,构建了pET一32a-VP1-VP4原核表达系统,以此获得VP1-VP4融合蛋白。制备骨髓源树突状细胞(BMDCs)和淋巴结T细胞,首先用清道夫受体抑制剂poly(I)处理.BMDCs,然后再将VPl-VP4.融合蛋白负载经poly(I)处理后的BMDCs,并与淋巴结T细胞共培养,收集不同时间点的共培养上清液,用ELISA检测其中IFN-γ含量。结果显示,经poly(I)处理的试验组在每个时间点产生的IFN-γ含量均明显高于对照组,且差异极显著(P<0.01)。表明清道夫受体可识别口蹄疫病毒VPl-VP4融合蛋白,并在BMDCs提呈VPl-VP4抗原的过程中发挥负调节作用。 The aim of this study was to investigate the role of the scavenger receptors on dendritic cells in presentation of recombinant VP1-VP4 of foot-and-mouth disease virus(FMDV) to T cells in vitro.The prokaryotic expression vector of pET-32a-VPl-VP4 was constructed and the VP1-VP4 protein was expressed and purified.Bone marrow-derived dendritic cells(BMDCs) pretreated with scavenger receptor inhibitors were pulsed with recombinant VP1-VP4 antigen and then cocultured with lymph node T cells.The culture supernatants were harvested at different time points for determining IFN-y contents with ELISA.The results showed that the IFN-y levels of experimental groups were significantly higher than that of control group at different time points(P<0.01).Our data indicate that scavenger receptors on BMDCs are able to recognize recombinant VP1-VP4 protein and play a negative regulatory role in antigen presentation of BMDCs to initiate lymph node T cell responses.

关 键 词： 口蹄疫病毒 融合蛋白 树突状细胞 抗原提呈 细胞

领　　域： [农业科学] [农业科学] [农业科学]