作　　者： ; ; ; ; ;

机构地区： 华南农业大学兽医学院

出　　处： 《中国预防兽医学报》 2004年第4期241-244,269,共5页

摘　　要： 以伪狂犬病病毒 (PRV)为载体表达其他病原体抗原蛋白是伪狂犬病疫苗研究的一个重要方向。本文以广东地方分离株 (以下简称YA)为亲本株 ,用PCR方法得到同源左右臂 ,然后将重组转移载体和PRVYA的基因组共转染 ,用BUDR筛选出了表达口蹄疫病毒 (Foot_and_MouthDiseaseVirus ,FMDV)结构蛋白VP1的TK_重组病毒 ,用PCR和SDS -PAGE的方法对VP1蛋白进行了初步鉴定 。 Pseudorabies (Aujeszky's disease),caused by Pseudorabies virus (PRV),is a major cause of sickness and death in young pigs and abortion in pregnant sows.Use of PRV as a system for expression of foreign antigens has been increasingly evaluated as an alternative to conventional vaccine production system.In this study, an isolate of PRV from Guangdong province (strain PRV YA) was used as parent strain. Some recombinant TK^- mutants expressing structural protein VP1 of FMDV (Foot-and-Mouth disease) were selected using BUDR (5-bromo-2-deoxy-uridine).The protein VP1 expressed in PRV TK^- mutants was identified primarily by SDS-PAGE and some biological characteristics of the recombinant mutants were studied.

关 键 词： 重组伪狂犬病病毒 基因缺失疫苗

领　　域： [农业科学] [农业科学] [农业科学]