作　　者： ; ; ; ; ; (何吉子）;

机构地区： 广东出入境检验检疫局

出　　处： 《食品安全质量检测学报》 2013年第1期245-250,共6页

摘　　要： 目的建立同时测定高脂肪食品中黄色2G、奶油黄、柑橘红2和苏丹红Ⅰ～Ⅳ的HPLC-DAD法。方法样品经环已烷/乙酸乙酯(1∶1,v/v)萃取,Bio-BeadsSX3凝胶层析柱净化去除油脂、天然色素等大分子干扰物质。采用C18色谱柱,以甲醇和酸性水溶液(pH4.0)为流动相梯度洗脱,二极管阵列检测器多波长同时检测这七种色素。结果在1.0～10.0mg/L范围内,峰面积与进样量呈良好的线性关系,线性相关系数大于0.999,回收率在81.0%~98.2%,相对标准偏差在0.89%~5.21%。黄色2G、奶油黄、柑橘红2的检出限均达到1.0mg/kg,苏丹红Ⅰ～Ⅳ色素的检出限均达到2.0mg/kg。结论该方法简便、快捷、灵敏、准确、重现性好,能满足相关色素同时检测的需要。 Objective To establish an HPLC-DAD method for simultaneous determination of yellow 2G, butter yellow, citrus red 2 and sudan red I^IV. Methods Samples were extracted directly with a mixture of cyclohexane-athylacetate (1∶1, v/v ) and purified by a Bio-beads SX3 gel column, to remove interfering high molecular fatty substances and natural pigments in food samples. HPLC was carried out on a C18 column with MeOH+H2O (pH 4.0) as mobile phase in the gradient elution. Multiwavelength diode array detection (DAD) was applied in the determination of the 7 kinds of pigments. Results Good linear relationships in the range of 1.0~10 mg/L of 7 compounds were obtained with correlation coefficients (r) all above 0.999. The RSDs were in the range of 0.89%~5.21%, and average recoveries were 81.0%~98.2%. The detection limits were 1.0 mg/kg for yellow 2G, butter yellow, citrus red 2 and 2.0 mg/kg for sudan red I^IV, respectively. Conclusion The established method is simple, rapid, sensitive, accurate and repeatable, and can be used for the simultaneous determination of relevant pigments.

关 键 词： 凝胶渗透色谱净化 高压液相色谱法 黄色 奶油黄 柑橘红 苏丹红 高脂肪食品

领　　域： [轻工技术与工程]