作　　者： ; ; ; ; ; ; ; ;

机构地区： 南方医科大学南方医院

出　　处： 《免疫学杂志》 2009年第3期314-317,共4页

摘　　要： 目的调查人类自然杀伤(NK)细胞表面免疫球蛋白样受体KIR在尿毒症患者的表达。方法采用PCR-SSP完成55例尿毒症患者KIR基因分析。结果尿毒症患者表达16个KIR基因,2DL4、3DL2、3DL3、3DP1基因频率1.00,2DL1、2DL3、3DL1、2DS4基因频率0.73～0.87,2DL2、2DS1、2DS2、2DS3、2DS5基因频率0.11～025,2DL5、3DS1、2DP1基因频率0.30～0.41。对尿毒症患者与部分亚洲地区人群的分布频率比较分析中发现,KIR-2DL4、3DL1、3DL2、3DL3、2DS4(GF0.73～1.00)和KIR-2DL2、2DL5、2DS1、2DS3、2DS5、3DS1(GF0.11～0.34)与韩国人、日本人、南亚人之间相差<0.20;KIR-2DL1、2DL3、2DS2与韩国人、日本人、南亚人群之间相差≥0.20,2DL1低于日本人,2DS2低于南亚人,2DL3高于南亚人。结论55例尿毒症患者普遍带有结构基因2DL4、3DL2、3DL3、3DP1,其次为2DL1、2DL3、3DL1、2DS4,较罕见为2DL2、2DS1、2DS2、2DS3和2DS5;显示尿毒症患者中14个KIR功能基因频率与亚洲其他人群KIR基因频率接近,但是KIR基因多态性与上述不同种族之间存在分布差异。 Objective To investigate the frequencies and polymorphism of killer cell immunoglobulin-like receptor(KIR) genes on the surface of NK cells in uremia patients. Methods Twenty-three pairs of primers were designed and different KIR genes in 55 random uremia patients were analyzed by PCR-SSP. Results Sixteen genes were observed in uremia patients. The frequencies (GF) of 2DL4,3DL2,3DL3 and 3DP1 were 1.00;GF of 2DL1,2DL3,3DL1,and 2DS4 were 0.73-0.87;GF of 2DL2,2DS1,2DS2,2DS3,and 2DS5 were 0.11-0.25;GF of 2DL5,3DS1,and 2DP1 were 0.30-0.41. Compared the frequencies of uremia patients (GF 0.73-1.00 in KIR-2DL4,3DL1,3DL2,3DL3,and 2DS4) with Korean,Japanese,Southern Asian subjects (GF 0.11～0.34 in KIR-2DL2,2DL5,2DS1,2DS3,2DS5,3DS1),the difference were less than 0.20. Therefore,the frequency of KIR-2DL1 in uremia patients was lower than that of Japanese,2DS2 was lower than that of Southern Asian,and 2DL3 was higher than that of Southern Asian. Conclusion All 16 known genes observed in uremia patients indicate that the KIR genes are polymorphic. The most frequent genes were 2DL4,3DL2,3DL3,and 3DP1; followed by 2DL1,2DL3,3DL1 and 2DS4; rare of 2DL2,2DS1,2DS2 ,2DS3 and 2DS5. This study shows that there is a high concordance but variability in the frequent distributions of KIR genes among the uremia patients and Asian populations.

关 键 词： 尿毒症 杀伤免疫球蛋白样受体 基因频率

领　　域： [生物学]