机构地区: 华中科技大学光电子科学与工程学院
出 处: 《深圳大学学报(理工版)》 2008年第4期376-380,共5页
摘 要: 在结构光显微系统中使用可编程数字微镜装置代替压电驱动的光栅,产生稳定可靠的结构光照明.为避免数字微镜装置本身的像素化结构对成像的影响,系统中引入一个低通的空间光调制器,以滤除经过数字微镜装置后产生的高级(±2nd及以上)衍射光.此时在样品上形成的结构光不再是单一频率的余弦光栅图案,传统结构光显微成像中通过光栅3步相移成像获得层析图像的方法不再适用.新算法通过5步相移得到5幅源图像,也可重构出层析图像.结果表明,传统荧光图像中几乎不能分辨的某些精细结构,在重构的层析图像中能够清晰地分辨出来. In our novel structured illumination microscope(SIM),a programmable digital micromirror device(DMD) was used instead of piezo activated grating to produce stable and reliable structured illumination.An optical low-pass spatial filter was applied to block the diffraction order higher than ±1st order.The illumination pattern was not a sinusoidal grid with a single spatial frequency,so the algorithm used in the traditional SIM is not proper here.An improved algorithm based on the five raw images with different...
领 域: [电子电信]