作　　者： ; ; ;

机构地区： 韶关学院英东生物工程学院

出　　处： 《韶关学院学报》 2008年第12期79-83,共5页

摘　　要： 采用基因工程方法,将PhiX174噬菌体裂解基因E和温敏控制表达系统与质粒pPBA1100基因杂交,构建了重组子pPBA1100-E.将重组子转化到多杀性巴氏杆菌中,通过温度诱导使裂解基因表达.用限制性内切酶检验重组子.扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率.结果表明:重组质粒经限制性内切酶酶切鉴定,琼脂糖电泳呈现三条谱带,相对分子质量与理论值一致.扫描电镜观察显示,重组子在多杀性巴氏杆菌中成功表达,获得了细菌幽灵.菌落形成单位检验结果显示,多杀性巴氏杆菌遗传灭活率达到99%. Recombinant pPBA1100-E was constructed by which the gene E of bacteriophage Phix174 and temperature sensitivity expressing control system hybridized with plasmid pPBA1100 by genetic engineering method.Recombinant was transform to Pasteurella multocida and lysis gene E expressed by temperature inducing.Recombinant was detected by restriction endonuclease.Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi...

关 键 词： 多杀性巴氏杆菌 细菌幽灵 裂解基因 质粒构建

领　　域： [农业科学] [农业科学] [农业科学]