作 者: ;
机构地区: 菏泽学院生命科学系
出 处: 《菏泽学院学报》 2008年第5期70-73,77,共5页
摘 要: 利用微卫星标记的共显性特点,选用5对微卫星引物对65个条斑紫菜叶状体个体的嵌合性,通过聚丙烯酰胺凝胶电泳进行了分析。结果显示:聚丙烯酰胺凝胶电泳中既有两条带又有一条带,也即条斑紫菜叶状体个体中既有嵌合体又有纯合体,其中58.5%为纯合体,41.5%为嵌合体。这为条斑紫菜叶状体的嵌合性提供了分子生物学方面的证据。 In order to provide the proof in molecular biology for the mosaic thalli of Porphyra yezoensis,the individual thallus is analyzed by the codominance of SSR.Sixty-five individuals of the thalli of P.yezoensis were analyzed with 5 microsatellite primers by PAGE electrophoresis.The results show that heterozygous individuals and pure individuals are both found with 41.5% are heterozygous,58.5% are pure.It increases the knowledge of mosaic thalli with cells of different genetic basis in P.yezoensis.During the ex...
领 域: [生物学]