作　　者： ; ;

机构地区： 韶关学院英东生物工程学院

出　　处： 《韶关学院学报》 2002年第9期70-76,共7页

摘　　要： 对蒙古黄芪无菌苗的不同外植体 (叶片、子叶、下胚轴 )进行组织培养研究 .结果表明 :该三种外植体分别在MS +BA2 0mg/L +NAA2 0mg/L、LS +BA2 0mg/L +NAA0 1mg/L、MS +BA2 0mg/L +NAA2 0mg/L培养基上的愈伤组织诱导率很高 ;在这三种外植体中 ,子叶和下胚轴所诱导的愈伤组织在继代培养基上能分化出芽 ;试管苗在 1/ 2MS +NAA5 In this experiment,a tissue culture research was made with the different parts of A.membranaceus Bge.var.mongholicus (Bge.) Hisao seedling as explants (leaf segment,cotyledon,hypocetyl). The results were as follows: the highest percentage of callus induction was different with different culture modium. The callus induced by the plants of cotyledon and hypocetyl may be produced shoot in successive transfer culture medium. The effect of rooting is best when transfering the unrooted tube seedling into 1/2MS(majar element 1/2) culture medium with NAA 5.0mg/L.

关 键 词： 蒙古黄芪 三种外植体 组织培养

领　　域： [生物学]